Friday, January 1, 2010

Kappa Casein Kappa-Casein Protein Name Origin?

Kappa-Casein Protein Name Origin? - kappa casein

What does the Kappa Kappa-casein (k-casein) proteins? I know it only a Greek letter, but why do we speak of Kappa and how?

Posted by Laura Meek at 8:59 PM

1 comment:

Eddie Roger Saluntao said...

A cDNA library was constructed with poly (A) + RNA from the mammary gland of cattle. This collection of cDNA clones of 6000, employment was screened by colony hybridization with 32P-labeled oligonucleotide probes and mapping of restriction endonucleases. The cDNA plasmid pKR76 chosen and sequenced by the dideoxy chain termination method. The insert cDNA pKR76 leads to full-length sequence that encodes the mature protein kappa-casein. The amino acid sequence derived from the DNA sequence corresponds to the published sequence of amino acids with three exceptions: the pyroglutamic reported in position 1, tyrosine at position 35 and aspartic acid at position 81 are each, glutamine, histidine, and asparagine in clone with pKR76. The MspI, cleaved NlaIV fragment (630 bp) of insert DNA kappa-casein was sub-divided into expression vectors pUC18 and pKK233-2, a promoter and a lac promoter containing cloned or CVR. Escherichia coli cells carrying out recombinant plasmid expression is shown to produce protein-kappa-casein than expected mobileING sodium dodecyl sulfate-polyacrylamide gel electrophoresis on and with specific antibodies are detected against natural bovine kappa-casein increased.

January 6, 2010 at 6:07 AM

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